Table 2 PCR conditions used for polymorphisms studied
|
XRCC5 (KU80) |
XRCC6 (KU70) |
XRCC7 (PRKDC) | |||||
|---|---|---|---|---|---|---|---|
| Temp | Time | Temp | Time | Temp | Time | ||
| Initial denaturation | 95 °C | 5 min | 95 °C | 5 min | 95 °C | 5 min | |
| Amplification (30 cycles) | Annealing | 60 °C | 30s | 58 °C | 30s | 63 °C | 30s |
| Extension | 72 °C | 30s | 72 °C | 30s | 72 °C | 30s | |
| Denaturation | 95 °C | 30s | 95 °C | 30s | 95 °C | 30s | |
| Final extension | 72 °C | 5 min | 72 °C | 5 min | 72 °C | 5 min | |